The information in this section is from caliper labchip gxgx ii user manual how the caliper software analyzes rna data. Date the tube and store filtered gel matrix in the dark. Quick and accurate analysis of rna and dna samples the labchip gx is the most accurate and advanced nucleic acid separations system available. We have a year 2016 pe labchip gx touch dnarna characterization system for sale. Ht rna labchip kit, version 2 labchip gxgxii user guide. After the labchip instrument optics detect the laserinduced fluorescent signal, easy to use system software automatically analyzes the data and provides the user. The phynexus shopping cart page employs the smartcart ecommerce software, in which users can easily navigate to search for products and order by credit card at any time. Primary data electropherograms are converted to virtual gels by the machine software. Equipnet is the worlds leading provider of used microplate readers and a number of other used equipment. Our antivirus scan shows that this download is clean. When powered on, the chip, eject, and status indicators labchip gxii light up. A novel by paul beatty pdf gidget the horse formerly known as. On the main screen, click on the run button in the upper left corner of the labchip gx software. Tracked stored samples using lims software laboratory integrated management system.
Caliper life sciences labchip gx automated electrophoresis w. Ht high sensitivity dna labchip kit, version 2 labchip gxgxii user guide am. The instruments ce 7100 by agilent technologies, labchip gxii touch ht by perkinelmer, maurice s. The gxii touch helps accelerate the development of biotherapeutic drugs by automating the protein characterization process. Add 4l ladder to 2l of pico 5x labeling buffer and 4l water. Blabchip gx touchgxii touch user manual perkinelmer, inc.
The caliper labchip gxii offers automated analysis, and quantitation of molecular weight, concentration and purity. To assist in your compliance planning, labchip gxp security software is available which allows the labchip gxii touch protein characterization system to run in an environment consistent with cfr21 part 11 compliance and. The labchip gxgxii electrophoresis systems use a single sipper microfluidic chip to aspirate samples directly from 96 or 384well plates. It is based on traditional gel electrophoresis, but it has a microfluidic chipbased format. The fragment sizes of the libraries were subsequently assessed by perkin elmer labchip gxii and agilent 2200 tapestation.
The labchip gxii is a complete solution for consistent and precise. Roan zaied msc student university of glasgow, college. High resolution labchip gxii, touch ht perkinelmer. The labchip gx software allows users to compare data. The labchip gx touch system provides rapid, quantifiable data for pre and postpcr sample analysis.
G3 ngsx workstation hardware, software, applications. Current listings 48 labx member since 23rd august, 2010. With complete analysis of genomic material in about 30 seconds, the labchip gx touch nucleic acid analyzer eliminates the nucleic acid quantitation workflow bottleneck. The software lies within education tools, more precisely science tools. Unless otherwise specified, prepare all master mixes and store all samples in a cold block or ice.
It operates via standard gel electrophoresis principles with microvolumes for sample acquisition times as low as 40 seconds. Chemical safety warning some chemicals used with the labchip gx touchgxii touch are potentially hazardous and can cause illness. Labchip small rna assay perkinelmer applied genomics. Laboratory methods in microfluidics basant giri auth. Labchip gx touch nucleic acid analyzer perkinelmer. View gibert kennedys profile on linkedin, the worlds largest professional community. Dna ngs 3k assay quick guide labchip gx touchgxii touch. Principles of operation on page 20 usage labchip gx touch software is for use with labchip gxgxii and gx touchgxii touch instruments. Labchip gx touch and gxii touch automated gel electrophoresis systems the labchip gx can enhance genomics research by precisely quantifying nucleic acids. The gx labchip was placed in a labchip gxii analyzer perkin elmer, 124582b and read using labchip gxii software. Lapchip gxii touch protein assay protfolio mediray.
Effects of antibody disulfide bond reduction on purification. Caliper labchip gxp software enables the users to run the instruments in regulated environments, such glp good laboratory practice, gcp good. Aliquot removed at each time pointplatform labchip gxii that performs multiple protein characterization assays on a variants was performed using the labchip gxii and a preproduction microfluidic herceptin 0, 1, 3, 8 days, labeled, and analyzed with labchip gxii. Protein express assay quick guide labchip gxii touch. Labchip gx touch microfluidics capillary electrophoresis by perkinelmer, inc labchip gx touch microfluidics technology offers unparalleled electrophoresis separation for high sensitivity dnarna analysis, dna smear analysis, and rna and gdna inte. When powered on, the chip, eject, and status indicators labchip gxii light up red. Integration overview integration with the perkinelmer labchip gxgxii is part of the standard qc package that is delivered with every edition of the lims. New features the following new assays are added and supported in. Pathways to the present 2002 physical chemistry a molecular approach answers pdf student safety sheets 55 bromine cleapss antibody analysis using calipers labchip gxii system sommerville software engineering 8th edition pdf pub.
Leveraging microfluidics technology the labchip gxii eliminates the need to handle time consuming sds page gels, and provides superior results for less time and money. Our exclusive contracts with our clients yield a wide range of used microplate readers from a number of respected oems, including perkin elmer, tecan, molecular devices, biotek instruments, packard automation, labsystems and more. The labchip gx rna assay analysis determines the quality of the rna sample by measuring the relative amounts of know rna fragments relative to the total rna present in the sample. Read perkinelmers applied genomics blog to learn how to improve the efficiency of your work in the applied genomics field. Product service and customer support plans perkinelmer offers a full range of services to ensure your success. Kostan,doran,delchevasay goodbye to gelsreal world use. Jul 01, 2012 labchip gxii separation is based on size, with the resolution ranging from 14 to 300 kda. The labchip gxii lcgxii platform shown in figure 1 provides an automated platform for rapid analysis of proteins and nucleic acids. The caliper life sciences labchip cxii is a chipbased platform for analyzing dna, rna, protein, and glycan assays. Assessment of genomic dna quality by microchip electrophoresis. Caliper life sciences labchip gx ii automated electrophoresis. The labchip touch platforms top differentiator is its ability to perform electrophoresis in microfluidic channels.
Discard filter and store filtered gel matrix in the dark at 4 o. Disentangling the drivers of functional complexity at the. Leveraging microfluidic electrophoretic separation technology, the labchip gxii touch system enables rapid characterization with minimal sample preparation setup and sample input volume. Sampling directly from a 96 or 384well plate, the lcgxii offers ease of use and walk away time. The instrument optics detect samples by laserinduced fluorescence. For analysis on the labchip, samples were first diluted 1. Automated sampling from 96 and 384well plates transfer plates from a thermal cycler directly into the labchip gx system, no sample prep or transfer needed. Pe labchip gx touch ht dnarna characterization system.
The labchip gx is designed for both rna and dna analysis, while the labchip gxii runs rna, dna, and also protein assays. Remove the chip from the chip storage container and inspect the chip window. The high resolution labchip is designed to be compatible with the glycan screening assay and low molecular weight protein assay on the labchip gxii microfluidic capillary electrophoresis cesds platform. Compatible software labchip gx touch software version 1. Read and understand the material safety data sheet msds provided by the chemical manufacturer before you store. If the enhanced functions are deactivated continue without pressing the insert ids button. The labchip gx touch software controls the labchip gx touch and labchip gxii touch instruments. Discard filter and store filtered gel matrix in the dark at 4oc. The labchip gxii touch protein characterization system from perkinelmer is a smart microfluidic platform for high throughput quantification and quality screening of protein molecules. Purchase orders, checks, and wire transfers are preferred. Credit card and paypal payments are accepted, but may be. See the complete profile on linkedin and discover giberts. A caliper labchip gxgxii instrument with labchip gxp software is a closed system 21 cfr 11. Gibert kennedy associate scientist ii biogen linkedin.
Highthroughput screening of mab charge variants using microchipcze authors. The labchip gxii touch protein characterization system provides the complete solution for reproducible quantitation, molecular weight sizing and percent purity analysis of protein samples. The gx labchip was placed in a labchip gxii analyzer perkin elmer. Labchip microchips and reagent kits are designed for multiple measurement up to 2,000 runs that fit modern highthroughput work flows. Labchip gx gxii user guide contents specifications 2 sample conditions 2. Use the lowthroughput protocol when running the labchip gxgxii touch 24 instrument.
Peaks that are resolved less than half height can still be accurately identified by the system software 2 higher salt concentrations and different ions may alter performance and reduce assay sensitivity. The kit is for research use only and should not be used in diagnostic procedures. Labchip gx touch microfluidics capillary electrophoresis by. The actual developer of the free software is perkinelmer inc. Labchip gxii touch ht protein characterization system perkinelmer.
The software contains an application security module for user account management and for access of controls to. Labchip gxii touch biotherapeutics how a chip works. The system can be located in a designated laboratory room to limit system access to authorized individuals 21 cfr 11. Chemical safety warning some chemicals used with the labchip gx touch gxii touch are potentially hazardous and can cause illness.
Labchip gx touch microfluidics for dnarna analysis. Other readers will always be interested in your opinion of the books youve read. After connecting a pc and opening the labchip software the status indicator. Rapid analysis of nglycans on the labchip gxii microchipce. When speed, accuracy and easeofuse are paramount, the labchip gxii is the perfect solution. The labchip small rna assay is compatible with both the labchip gx touch nucleic acid analyzer and the labchip gxii touch protein characterization system. Highthroughput screening of mab charge variants using. The next generation of capillary electrophoresis instruments. Perkinelmer labchip gxgxii genologics, an illumina company. Chip in the labchip gx touch gxii touch instrument. Used caliper life sciences labchip gxii 2035202 dna.
From our original factory warranty through a comprehensive line of customer support plans, perkinelmer offers you field service engineers and inhouse specialists who are. Add 5l of 40m reconstituted dye in water to 5l of denatured ladder. Intuitive touch screen interface with comprehensive analysis using labchip gx software enabling true highthroughput analytics. Higherthroughput protein production and characterization. Result values from the labchip gxgxii can be parsed into the lims. Jun 15, 2011 the labchip gxgxii systems offer researchers an automated alternative by streamlining the multiple, manual steps of slab gel electrophoresis, while also providing the throughput and data quality. Analysis of each sample is fast, occurring in 40 sec, and the samples are analyzed directly from a 96well plate. Each individual sample can be analyzed in 41 seconds. Caliper life sciences labchip gx automated electrophoresis. Ht rna labchip kit, version 2 labchip gxgxii user guide labchip gx rna assay quick guide im. After connecting a pc and opening the labchip software the status indicator turns green. This assay incorporates a percent purity reference standard with automated calibration and optimization software.
Tobias wheeler, lucy sun, rajendra singh, bahram fathollahi and hans pirard caliper a perkinelmer company, alameda, ca, usa intermediate assay reproducibility relative areas of basic, main, and acidic variants 1 abstract. In cambridge healthtech institutes higherthroughput protein production and characterization conference, htp is explored in the quest to develop methods that ensure quality and translate to large scale much more quickly and efficiently than in the past. Perkinelmer labchip gx touch user manual pdf download. As part of the qc package, the lims includes support for. Chemical safety warning some chemicals used with the labchip dx are potentially hazardous and can cause illness. Inserting a chip into the labchip gxgxii touch instrument 1 2 check that the sample plate, buffer tube, and ladder tube are placed on the instrument properly. Labchip gxgxii and gx touchgxii touch instruments are for research use only and not for use in diagnostic procedures. Determination of protein charge variants with imaged. Blabchip gx touch gxii touch user manual perkinelmer, inc. Operated laboratory machinery including hamilton for blood fractionation and rnadna extraction, flow cytometer to asses cellular viability, dxh 500, brooks automated biostore, qubit fluorometer, perkinelmer labchip gxii, etc. The software then shows an alert warning barcode reader disconnected and no chip. Dna ngs 3k labchips item part number ht xmark labchip for use with gx touchgxii touch ht cls144006 24 xmark labchip for. Ht dna high sensitivity labchip kit labchip gxgxii user.
Store the chip according to the instructions in the assay user guide. Guangnan meng, product manager, microfluidics, perkinelmer. Labchip ez reader supports 4sipper chips and is designed for compound profiling using profilerpro kit plates. If you dont see the used microplate reader that youre.
Mock served for nearly 20 years in a wide range of financial oversight capacities within general electric company ge. The start run dialog box will pop up with tabs listed as output, run and advanced. System requirements for labchip gx software instrument mode labchip gx. The labchip gx software provides an optical calibration function using test chip c to calculate a correction factor for each individual labchip gx instrument. On the main screen, click the run button in the upper left corner of the labchip gx software. The diagnostic menu within the labchip software is also. In this method pngase freleased nglycans in a 96well microtiter plate are fluores.
Labchip gxii touch ht protein characterization system. Follow the instructions as given in the chemagic qa software. Operation and maintenance of the caliper labchip gx for dna. There was a 4 log linear range of detection lower limit of detection was about 10 pg. Log on to nimbus and start the hamilton run control software. Highthroughput screening of mab charge variants using microchipcze 1. Labchip gxii touch biotherapeutics how a chip works youtube. The plate and a chip that contained the gel dye, the destain solution, and the protein express lower maker were placed into a labchip gx system perkin elmer, waltham, ma, 124582 for analysis.
Highresolution analysis provides exact sizing and quantitation of dna fragments and smears down to 5 bp from samples as small as 2 pgl. A high throughput capillary electrophoresis method to obtain. Rapid analysis of nglycans on the labchip gxii microchipce platform abstract a high throughput microchipce method on the labchip gxii has been developed for profiling nglycans from recombinant igg antibodies. Whether youve loved the book or not, if you give your.
Caliper life sciences the caliper life sciences labchip gx was designed to target genomic applications and research for analysis of rna integrity. Labchip gxii touch protein characterization system. Dna ngs 3k labchips item part number ht xmark labchip for use with gx touchgxii touch ht cls144006 24 xmark labchip for use with gx touchgxii touch 24 cls145331. Labchip gx assays assessment of genomic dna quality by microchip electrophoresis introduction as the study of disease and drug response at the genetic level becomes more commonplace, the availability of highquality genomic dna gdna and a reliable method to assess this quality are essential. Here we demonstrate the utility of the labchip gxii touch microfluidic system in applying microfluidic electrophoresis technology to protein impurity analysis using the protein clear hr assay. The correction factor is applied to all samples run on the instrument to provide a common absolute fluorescence across different instruments. Software version found by choosing about from the main help menu.